Create kendoUpload:

var ku = $('#file').kendoUpload({});


Destroy it:
$('#file').data('kendoUpload').destroy();

Create again with files option:
var ku = $('#file').kendoUpload({
     files: [{name: 'name', size: '5000', extension: '.jpg'}]
});

and try to remove file by click on cross, you'll get exception:

Uncaught TypeError: Cannot call method 'remove' of undefined kendo.web.js:47077