If a column does not have width set, it will not populate the Columns collection of the ExportInfrastructure in the BiffExprorting event http://docs.telerik.com/devtools/aspnet-ajax/controls/grid/functionality/exporting/export-formats/excel-biff-export To be able to loop through all columns, you need to have width specified for them. This change has been introduced in Q3 2014 If you cannot do this for the standard grid rendering when defining columns, you can use the Grid.PreRender event to loop all columns and set some width for them: VB Protected Sub RadGrid1_PreRender(ByVal sender As Object, ByVal e As System.EventArgs) Handles RadGrid1.PreRender 'workaround for BiffExporting event Columns collection not being populated if the column does not have width set Dim Grid As RadGrid = DirectCast(sender, RadGrid) If (Grid.IsExporting) Then For index = 0 To Grid.MasterTableView.Columns.Count - 1 Grid.MasterTableView.Columns(index).HeaderStyle.Width = 100 Next End If End Sub C# protected void RadGrid1_PreRender(object sender, System.EventArgs e) { //workaround for BiffExporting event Columns collection not being populated if the column does not have width set RadGrid Grid = (RadGrid)sender; if ((Grid.IsExporting)) { for (index = 0; index <= Grid.MasterTableView.Columns.Count - 1; index++) { Grid.MasterTableView.Columns(index).HeaderStyle.Width = 100; } } }