Currently it is not possible to add null-able series items in RadHtmlChart in the markup or programmatically. If a null-able item is added it is omitted and is replaced by the next series item from the current Series. The workaround is to either data bound the chart to a data source or convert the null-able values to zeros:
C#:
        decimal? nullValue = null;
        if (nullValue == null)
        {
            nullValue = 0;
        }
        ColumnSeries colSeries2 = RadHtmlChart1.PlotArea.Series[1] as ColumnSeries;
        colSeries2.SeriesItems.Add(39);
        colSeries2.SeriesItems.Add(nullValue);
        colSeries2.SeriesItems.Add(3);