Let's work on your case in your private ticket first, because it seems you are checking all components so the problem you are facing might be different.
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iisnode encountered an error when processing the request.
HTTP status: 500
HTTP subStatus: 1013
HTTP reason: Internal Server Error
You are receiving this HTTP 200 response because system.webServer/iisnode/@devErrorsEnabled configuration setting is 'true'.
In addition to the log of stdout and stderr of the node.exe process, consider using debugging and ETW traces to further diagnose the problem.
The last 64k of the output generated by the node.exe process to stderr is shown below:
(node:7804) [DEP0005] DeprecationWarning: Buffer() is deprecated due to security and usability issues. Please use the Buffer.alloc(), Buffer.allocUnsafe(), or Buffer.from() methods instead.